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Proteintech mouse monoclonal anti per2 antibody

Figure 1. <t>PER2</t> knockdown by PER2-shRNA in SCC15 cells. (A) Levels of PER2 mRNA were significantly reduced in SCC15 cells transfected with PER2-shRNA-I-III, and the levels of PER2 mRNA were significantly decreased in the PER2-shRNA-I group as compared to the PER2-shRNA-II and PER2-shRNA-III groups. (B) Gel images of PER2 protein level analyzed by western blotting in the SCC15, Control-shRNA, and PER2-shRNA-I-III groups. (C) Expression of PER2 protein was significantly downregulated in SCC15 cells transfected with PER2-shRNA-I, PER2-shRNA-II, or PER2-shRNA-III. Means ± SD from three independent experiments are shown. Significant differences among multiple groups were evaluated using one-way ANOVA; differ- ences between two groups were evaluated using the LSD test. Statistical significance is indicated by asterisks. ***P<0.001.

Mouse Monoclonal Anti Per2 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+monoclonal+anti+per2+antibody/PER2+Antibody/pm28535015-65-16-56
Average 94 stars, based on 49 article reviews

mouse monoclonal anti per2 antibody - by Bioz Stars, 2026-10

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1) Product Images from "The circadian clock gene PER2 plays an important role in tumor suppression through regulating tumor-associated genes in human oral squamous cell carcinoma."

Article Title: The circadian clock gene PER2 plays an important role in tumor suppression through regulating tumor-associated genes in human oral squamous cell carcinoma.

Journal: Oncology reports

doi: 10.3892/or.2017.5653

Figure 1. PER2 knockdown by PER2-shRNA in SCC15 cells. (A) Levels of PER2 mRNA were significantly reduced in SCC15 cells transfected with PER2-shRNA-I-III, and the levels of PER2 mRNA were significantly decreased in the PER2-shRNA-I group as compared to the PER2-shRNA-II and PER2-shRNA-III groups. (B) Gel images of PER2 protein level analyzed by western blotting in the SCC15, Control-shRNA, and PER2-shRNA-I-III groups. (C) Expression of PER2 protein was significantly downregulated in SCC15 cells transfected with PER2-shRNA-I, PER2-shRNA-II, or PER2-shRNA-III. Means ± SD from three independent experiments are shown. Significant differences among multiple groups were evaluated using one-way ANOVA; differ- ences between two groups were evaluated using the LSD test. Statistical significance is indicated by asterisks. ***P<0.001.

Figure Legend Snippet: Figure 1. PER2 knockdown by PER2-shRNA in SCC15 cells. (A) Levels of PER2 mRNA were significantly reduced in SCC15 cells transfected with PER2-shRNA-I-III, and the levels of PER2 mRNA were significantly decreased in the PER2-shRNA-I group as compared to the PER2-shRNA-II and PER2-shRNA-III groups. (B) Gel images of PER2 protein level analyzed by western blotting in the SCC15, Control-shRNA, and PER2-shRNA-I-III groups. (C) Expression of PER2 protein was significantly downregulated in SCC15 cells transfected with PER2-shRNA-I, PER2-shRNA-II, or PER2-shRNA-III. Means ± SD from three independent experiments are shown. Significant differences among multiple groups were evaluated using one-way ANOVA; differ- ences between two groups were evaluated using the LSD test. Statistical significance is indicated by asterisks. ***P<0.001.

Techniques Used: Knockdown, shRNA, Transfection, Western Blot, Control, Expressing

Figure 2. Regulation of mRNA expression of tumor-related genes by silenced PER2 in SCC15 cells. The levels of Ki-67, c-Myc, Bcl-2, MDM2, VEGF, MMP2, TIMP-2, p53, and BaxmRNA were determined by real-time PCR in PER2-shRNA-I, Control-shRNA, and SCC15 groups. Means ± SD from three independent experiments are shown. Significant differences among multiple groups were evaluated using one-way ANOVA; differences between two groups were evaluated using the LSD test. Statistical significance is indicated by asterisks. **P<0.01, ***P<0.001.

Figure Legend Snippet: Figure 2. Regulation of mRNA expression of tumor-related genes by silenced PER2 in SCC15 cells. The levels of Ki-67, c-Myc, Bcl-2, MDM2, VEGF, MMP2, TIMP-2, p53, and BaxmRNA were determined by real-time PCR in PER2-shRNA-I, Control-shRNA, and SCC15 groups. Means ± SD from three independent experiments are shown. Significant differences among multiple groups were evaluated using one-way ANOVA; differences between two groups were evaluated using the LSD test. Statistical significance is indicated by asterisks. **P<0.01, ***P<0.001.

Techniques Used: Expressing, Real-time Polymerase Chain Reaction, shRNA, Control

Figure 3. PER2 downregulation promotes cell growth and proliferation. (A) Cell Counting Kit-8 (CCK-8) assay. (B) Histograms show the number of colonies. (C) Colony formation assay. Means ± SD from three independent experiments are shown. Significant differences among multiple groups were evaluated using one-way ANOVA; differences between two groups were evaluated using the LSD test. Statistical significance is indicated by asterisks. *P<0.05, ***P<0.001.

Figure Legend Snippet: Figure 3. PER2 downregulation promotes cell growth and proliferation. (A) Cell Counting Kit-8 (CCK-8) assay. (B) Histograms show the number of colonies. (C) Colony formation assay. Means ± SD from three independent experiments are shown. Significant differences among multiple groups were evaluated using one-way ANOVA; differences between two groups were evaluated using the LSD test. Statistical significance is indicated by asterisks. *P<0.05, ***P<0.001.

Techniques Used: Cell Counting, CCK-8 Assay, Colony Assay

Figure 4. Effects of PER2 downregulation on cell cycle distribution, cell proliferation, and cell apoptosis in the SCC15 cells. (A) Flow cytometric analysis of the cell cycle. (B) Flow cytometric analysis of cell apoptosis. (C) The proportion of cells in G0/G1 phase, S phase, and G2/M phase to the total number of cells; proliferation index (PI) and apoptosis index (AI) in the three groups. Means ± SD from three independent experiments are shown. Significant differences among multiple groups were evaluated using one-way ANOVA; differences between two groups were evaluated using the LSD test. Statistical significance is indicated by asterisks. *P<0.05, **P<0.01.

Figure Legend Snippet: Figure 4. Effects of PER2 downregulation on cell cycle distribution, cell proliferation, and cell apoptosis in the SCC15 cells. (A) Flow cytometric analysis of the cell cycle. (B) Flow cytometric analysis of cell apoptosis. (C) The proportion of cells in G0/G1 phase, S phase, and G2/M phase to the total number of cells; proliferation index (PI) and apoptosis index (AI) in the three groups. Means ± SD from three independent experiments are shown. Significant differences among multiple groups were evaluated using one-way ANOVA; differences between two groups were evaluated using the LSD test. Statistical significance is indicated by asterisks. *P<0.05, **P<0.01.

Techniques Used:

Figure 5. PER2 downregulation promotes cell migration and invasive ability. (A) Transwell migration assays were performed in PER2-shRNA-I, Control- shRNA, and SCC15 groups. Histograms show the number of SCC15 cells migrating through the inserts. (B) Transwell invasion assays were performed in PER2-shRNA-I, Control-shRNA, and SCC15 groups. Histograms show the number of SCC15 cells invading through the inserts coated with Matrigel. Means ± SD from three independent experiments are shown. Significant differences among multiple groups were evaluated using one-way ANOVA; differ- ences between two groups were evaluated using the LSD test. Statistical significance is indicated by asterisks. ***P<0.001.

Figure Legend Snippet: Figure 5. PER2 downregulation promotes cell migration and invasive ability. (A) Transwell migration assays were performed in PER2-shRNA-I, Control- shRNA, and SCC15 groups. Histograms show the number of SCC15 cells migrating through the inserts. (B) Transwell invasion assays were performed in PER2-shRNA-I, Control-shRNA, and SCC15 groups. Histograms show the number of SCC15 cells invading through the inserts coated with Matrigel. Means ± SD from three independent experiments are shown. Significant differences among multiple groups were evaluated using one-way ANOVA; differ- ences between two groups were evaluated using the LSD test. Statistical significance is indicated by asterisks. ***P<0.001.

Techniques Used: Migration, shRNA, Control

Figure 6. PER2 downregulation increased SCC15 cell growth in vivo. (A) Xenograft that originated from PER2-shRNA-I cells grew larger than SCC15‑derived tumors. (B) Hematoxylin and eosin (H&E) staining of tissues (magnification, x200). Mean ± SD from five independent experiments are shown. Scale bar, 50 µm. Statistical significance is indicated by asterisks. ***P<0.001 (t-test).

Figure Legend Snippet: Figure 6. PER2 downregulation increased SCC15 cell growth in vivo. (A) Xenograft that originated from PER2-shRNA-I cells grew larger than SCC15‑derived tumors. (B) Hematoxylin and eosin (H&E) staining of tissues (magnification, x200). Mean ± SD from five independent experiments are shown. Scale bar, 50 µm. Statistical significance is indicated by asterisks. ***P<0.001 (t-test).

Techniques Used: In Vivo, shRNA, Staining

Incubation:

Article Title: Circadian clock gene Per2 plays an important role in cell proliferation, apoptosis and cell cycle progression in human oral squamous cell carcinoma.
Article Snippet: Proteins (50 μg) were seperated by 8% SDS-PAGE using Mini-PRoTEAN 3 system (Bio-Rad, Hercules, CA, USA) and transferred to polyvinylidene difluoride (PVDF) membranes (Pierce, Rockford, IL, USA) using Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad). .. The membranes were incubated with mouse monoclonal anti-Per2 antibody (1:500; 19-J6:sc-101105; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) and mouse monoclonal anti-β-actin antibody (1:1,000; 60008-1-lg), respectively, overnight at 4 ̊C, followed by goat monoclonal anti-mouse IgG (1:1,000) (SA00001-1) (both from ProteinTech, Chicago, IL, USA) for 1 h at 37 ̊C. .. Blots were detected using enhanced chemiluminescence reagent (Pierce) under a fluorescent chemiluminescence imaging system (ChemiDoc XRS+; Bio-Rad).

Article Title: The circadian clock gene PER2 plays an important role in tumor suppression through regulating tumor-associated genes in human oral squamous cell carcinoma.
Article Snippet: Proteins (50 μg) were separated by SDS-PAGE (6%) gel for electrophoresis and transferred to polyvinylidene difluoride (PVDF) membranes (Pierce, Rockford, IL, uSA). .. The membranes were blocked with 5% skim milk and subsequently incubated overnight at 4 ̊C with mouse monoclonal anti-PER2 antibody (1:500; 19-J6:sc-101105; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, uSA) and mouse monoclonal anti-β-actin antibody (1:1000; 60008-1-1g; Santa Cruz Biotechnology, Inc.), respectively, washed three times in PBS, followed by secondary goat monoclonal anti-mouse IgG (1:1000; SA00001-1; Protein Tech, Chicago, IL, uSA) for 1 h at room temperature. .. The precipitated proteins were washed three times in PBS and then detected and photographed by an ECL-advance Western Blot Detection System (Chmi Doc XRS+, Bio-Rad Laboratories, Inc., Hercules, CA, uSA).

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Journal: Oncology reports

Article Title: The circadian clock gene PER2 plays an important role in tumor suppression through regulating tumor-associated genes in human oral squamous cell carcinoma.

doi: 10.3892/or.2017.5653

Figure Lengend Snippet: Figure 1. PER2 knockdown by PER2-shRNA in SCC15 cells. (A) Levels of PER2 mRNA were significantly reduced in SCC15 cells transfected with PER2-shRNA-I-III, and the levels of PER2 mRNA were significantly decreased in the PER2-shRNA-I group as compared to the PER2-shRNA-II and PER2-shRNA-III groups. (B) Gel images of PER2 protein level analyzed by western blotting in the SCC15, Control-shRNA, and PER2-shRNA-I-III groups. (C) Expression of PER2 protein was significantly downregulated in SCC15 cells transfected with PER2-shRNA-I, PER2-shRNA-II, or PER2-shRNA-III. Means ± SD from three independent experiments are shown. Significant differences among multiple groups were evaluated using one-way ANOVA; differ- ences between two groups were evaluated using the LSD test. Statistical significance is indicated by asterisks. ***P<0.001.

Article Snippet: The membranes were blocked with 5% skim milk and subsequently incubated overnight at 4 ̊C with mouse monoclonal anti-PER2 antibody (1:500; 19-J6:sc-101105; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, uSA) and mouse monoclonal anti-β-actin antibody (1:1000; 60008-1-1g; Santa Cruz Biotechnology, Inc.), respectively, washed three times in PBS, followed by secondary goat monoclonal anti-mouse IgG (1:1000; SA00001-1; Protein Tech, Chicago, IL, uSA) for 1 h at room temperature.

Techniques: Knockdown, shRNA, Transfection, Western Blot, Control, Expressing

Journal: Oncology reports

Article Title: The circadian clock gene PER2 plays an important role in tumor suppression through regulating tumor-associated genes in human oral squamous cell carcinoma.

doi: 10.3892/or.2017.5653

Figure Lengend Snippet: Figure 2. Regulation of mRNA expression of tumor-related genes by silenced PER2 in SCC15 cells. The levels of Ki-67, c-Myc, Bcl-2, MDM2, VEGF, MMP2, TIMP-2, p53, and BaxmRNA were determined by real-time PCR in PER2-shRNA-I, Control-shRNA, and SCC15 groups. Means ± SD from three independent experiments are shown. Significant differences among multiple groups were evaluated using one-way ANOVA; differences between two groups were evaluated using the LSD test. Statistical significance is indicated by asterisks. **P<0.01, ***P<0.001.

Techniques: Expressing, Real-time Polymerase Chain Reaction, shRNA, Control

Journal: Oncology reports

Article Title: The circadian clock gene PER2 plays an important role in tumor suppression through regulating tumor-associated genes in human oral squamous cell carcinoma.

doi: 10.3892/or.2017.5653

Figure Lengend Snippet: Figure 3. PER2 downregulation promotes cell growth and proliferation. (A) Cell Counting Kit-8 (CCK-8) assay. (B) Histograms show the number of colonies. (C) Colony formation assay. Means ± SD from three independent experiments are shown. Significant differences among multiple groups were evaluated using one-way ANOVA; differences between two groups were evaluated using the LSD test. Statistical significance is indicated by asterisks. *P<0.05, ***P<0.001.

Techniques: Cell Counting, CCK-8 Assay, Colony Assay

Journal: Oncology reports

Article Title: The circadian clock gene PER2 plays an important role in tumor suppression through regulating tumor-associated genes in human oral squamous cell carcinoma.

doi: 10.3892/or.2017.5653

Figure Lengend Snippet: Figure 4. Effects of PER2 downregulation on cell cycle distribution, cell proliferation, and cell apoptosis in the SCC15 cells. (A) Flow cytometric analysis of the cell cycle. (B) Flow cytometric analysis of cell apoptosis. (C) The proportion of cells in G0/G1 phase, S phase, and G2/M phase to the total number of cells; proliferation index (PI) and apoptosis index (AI) in the three groups. Means ± SD from three independent experiments are shown. Significant differences among multiple groups were evaluated using one-way ANOVA; differences between two groups were evaluated using the LSD test. Statistical significance is indicated by asterisks. *P<0.05, **P<0.01.

Techniques:

Journal: Oncology reports

Article Title: The circadian clock gene PER2 plays an important role in tumor suppression through regulating tumor-associated genes in human oral squamous cell carcinoma.

doi: 10.3892/or.2017.5653

Figure Lengend Snippet: Figure 5. PER2 downregulation promotes cell migration and invasive ability. (A) Transwell migration assays were performed in PER2-shRNA-I, Control- shRNA, and SCC15 groups. Histograms show the number of SCC15 cells migrating through the inserts. (B) Transwell invasion assays were performed in PER2-shRNA-I, Control-shRNA, and SCC15 groups. Histograms show the number of SCC15 cells invading through the inserts coated with Matrigel. Means ± SD from three independent experiments are shown. Significant differences among multiple groups were evaluated using one-way ANOVA; differ- ences between two groups were evaluated using the LSD test. Statistical significance is indicated by asterisks. ***P<0.001.

Techniques: Migration, shRNA, Control

Journal: Oncology reports

Article Title: The circadian clock gene PER2 plays an important role in tumor suppression through regulating tumor-associated genes in human oral squamous cell carcinoma.

doi: 10.3892/or.2017.5653

Figure Lengend Snippet: Figure 6. PER2 downregulation increased SCC15 cell growth in vivo. (A) Xenograft that originated from PER2-shRNA-I cells grew larger than SCC15‑derived tumors. (B) Hematoxylin and eosin (H&E) staining of tissues (magnification, x200). Mean ± SD from five independent experiments are shown. Scale bar, 50 µm. Statistical significance is indicated by asterisks. ***P<0.001 (t-test).

Techniques: In Vivo, shRNA, Staining

Figure 2. PER2 mRNA expression (line graph) and PER2 protein expression (bar graph) in acute ischemic heart disease (AIHD).

Journal: Journal of Hard Tissue Biology

Article Title: Role of Circadian Clock Genes in Sudden Cardiac Death: A Pilot Study

doi: 10.2485/jhtb.26.347

Figure Lengend Snippet: Figure 2. PER2 mRNA expression (line graph) and PER2 protein expression (bar graph) in acute ischemic heart disease (AIHD).

Article Snippet: After three washes with PBS for 5 min, specimens were incubated overnight with the following primary antibodies at empirically determined dilutions: rabbit anti-human BMAL1 polyclonal antibody (sc-48790, 1:50; Santa Cruz Biotechnology), mouse anti-human PER2 monoclonal antibody (sc-101105, 1:50; Santa Cruz Biotechnology)33), mouse anti-human REV-ERBα monoclonal antibody (119-14508, 1:100; Ray Biotech)34).

Techniques: Expressing

Figure 5. PER2 mRNA expression (line graph) and PER2 protein expression (bar graph) in acute myocardial infarction (AMI).

Journal: Journal of Hard Tissue Biology

Article Title: Role of Circadian Clock Genes in Sudden Cardiac Death: A Pilot Study

doi: 10.2485/jhtb.26.347

Figure Lengend Snippet: Figure 5. PER2 mRNA expression (line graph) and PER2 protein expression (bar graph) in acute myocardial infarction (AMI).

Techniques: Expressing

Figure 8. PER2 mRNA expression (line graph) and PER2 protein expression (bar graph) in recurrent myocardial infarction (RMI).

Journal: Journal of Hard Tissue Biology

Article Title: Role of Circadian Clock Genes in Sudden Cardiac Death: A Pilot Study

doi: 10.2485/jhtb.26.347

Figure Lengend Snippet: Figure 8. PER2 mRNA expression (line graph) and PER2 protein expression (bar graph) in recurrent myocardial infarction (RMI).

Techniques: Expressing

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mouse monoclonal anti per2 antibody (Proteintech)

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